Effect of Fortification and Processing on Antinutrients, Protein Content, and Digestibility of Low-and High-Tannin Sorghum Cultivars

Eman A. Abdallah

Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan.

Almujtaba H. M. Abdallh

Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan.

Elfadil E. Babiker *

Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan and Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia.

*Author to whom correspondence should be addressed.


Abstract

This study aimed to examine the effects of wheat and pigeon pea fortification and processing on antinutrient levels, protein content, and in vitro protein digestibility (IVPD) of low-tannin (Tabat) and high-tannin (WadAhmed) sorghum cultivars. The sorghum cultivar WadAhmed exhibits elevated tannin (960.47 mg/100 gm) and phytate (265.34 mg/100 gm) concentrations and reduced protein content (10.41%) compared to other ingredients. Pigeon pea exhibited a markedly elevated IVPD (65.15%) value, while wheat demonstrated lower levels of tannins (41.47 mg/100 gm) and phytates (44.12 mg/100 gm) than other ingredients. The tannin, phytate, and protein contents, as well as the IVPD of the meals, were evaluated before and after processing. The incorporation of wheat and pigeon pea flours reduced antinutrient levels and increased protein content and in vitro protein digestibility (IVPD) of the meals. Post-fermentation, antinutrient levels were markedly reduced (P ≤ 0.05), coinciding with a considerable increase in IVPD for all meals. Cooking the fermented dough of meals showed significantly greater improvements in protein content, digestibility, and antinutrient removal than cooking raw meals. The blending of ingredients and fermentation diminished the impact of cooking on protein content and IVPD of all meals.

Keywords: Sorghum, fortification, kisra processing, antinutrients, in vitro protein digestibility


How to Cite

Abdallah, Eman A., Almujtaba H. M. Abdallh, and Elfadil E. Babiker. 2026. “Effect of Fortification and Processing on Antinutrients, Protein Content, and Digestibility of Low-and High-Tannin Sorghum Cultivars”. European Journal of Nutrition & Food Safety 18 (4):35-48. https://doi.org/10.9734/ejnfs/2026/v18i41997.

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