Jatropha tanjorensis Euphorbiaceae Ethanolic Leaves Extract Reverses Phenylhydrazine Induced Haematological Alterations in Albino Wistar Rats
E. B. Umoren *
Department of Physiology, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
I. A. Okon
Department of Physiology, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
C. Nyenke
Department of Medical Laboratory Sciences, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
E. Modo
Department of Biochemistry, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
N. Amadi
Department of Physiology, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
P. I. Brown
Department of Physiology, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Phenylhydrazine has been used in many studies to evaluate its modulatory effects in various biochemical parameters in whole blood and red blood cell lysate. Jatropha tanjorensis Euphorbiaceae have high antioxidants properties; its leaves phytochemical analysis shows the presence of flavonoids, tanins, terpenoids, saponis. This study investigated the ameliorative effects of Jatropha tanjorensis Euphorbiaceae on phenylhydrazine induced haematological alterations in albino Wistar rats.
Materials and Methods: Wistar rats of both sexes (180-200g) were divided into 4 groups (n=5). Group 1 received rat chow; Group 2 received (200 mg/kg) of J. tanjorensis orally. Group 3 received phenylhydrazine only (10 mg/kg). Group 4 received phenylhydrazine (10 mg/kg) + J. tanjorensis (250 mg/kg). All animals were allowed free access to clean drinking water and normal rat chow ad libitum for 35 days. After which animals were sacrificed and blood samples collected for biochemical analysis.
Results: Results obtained showed that phenylhydrazine induced normochromic anemia with significant increase in white blood cell count, and neutrophil counts, eosinophils (insignificant) count with a significant reduction in lymphocyte count. However, J. tanjorensis extract reversed the adverse haematological changes induced by phenylhydrazine.
Conclusion: In conclusion, Jatropha tanjorensis Euphorbiaceae demonstrated antioxidant, anti-inflammatory, and anti-thrombotic effects and reversed the haematological alterations brought upon by phenylhydrazine administration.
Keywords: Anemia, anti-inflammatory, Jatropha tanjorensis, phenylhydrazine, Wistar rats