Antioxidant, Mineral and Microbiological Properties of Wine from Blends of Roselle Calyces Extract and Pineapple Juice

Main Article Content

M. T. Ukeyima
B. O. Idoko
L. O. Moghalu


Wine was produced from must formulated by mixing roselle calyces hot water extract with pineapple juice at ratios of 100:0, 90:10, 80:20, 70:30, 60:40, 50:50 for A, B, C, D, E and F respectively. Must were pitched with Saccharomyces cerevisiae, fermented at room temperature for 8 days followed by racking, pasteurization and ageing at room temperature for 31 days. The antioxidant capacity, mineral content and microbiological analyses were carried out on the musts and wine samples using standard procedures. There was a significant (p<0.05) difference between must and wines as fermentation was observed to increase its antioxidant capacity (2, 2-diphenyl -1- picrylhydrazylradical scavenging activity, ferric reducing antioxidant properties, trolox equivalent antioxidant properties and hydroxyl radical scavenging activity). Fermentation decreased the mineral contents (manganese, zinc and magnesium) of wines except for sodium that increased significantly. Values ranged from 3.81 – 7.77 mg/L for Manganese, 162.38 – 166.66 mg/L for sodium, 2.71 – 4.71 mg/L for zinc and 35.45 – 40.67 mg/L for magnesium. Microbial count was done at different stages of production and there was no detectable growth on cultured wine samples. The result of this study showed that wines of nutritionally high quality can be produced from blends of roselle hot water extract and pineapple juice.

Wine, roselle calyces extracts, pineapple juice, antioxidant, minerals

Article Details

How to Cite
Ukeyima, M. T., Idoko, B. O., & Moghalu, L. O. (2020). Antioxidant, Mineral and Microbiological Properties of Wine from Blends of Roselle Calyces Extract and Pineapple Juice. European Journal of Nutrition & Food Safety, 12(7), 49-57.
Original Research Article


Duarte WF, Dias DR, Oliveira JM, Vilanova M, Teixeira JA, e Silva JBA, Schwan RF. Raspberry (Rubus idaeus L.) wine: Yeast selection, sensory evaluation and instrumental analysis of volatile and other compounds. Food Research International. 2010;43(9):2303-2314.

Okeke BC, Agu KC, Uba PO, Awah NS, Anaukwu CG, Archibong EJ, Orji MU. Wine production from mixed fruits (Pineapple and Watermelon) using high alcohol tolerant yeast isolated from palm wine. Universal Journal of Microbiology Research. 2015;3(4):41-45.

Swami SB, Thakor NJ, Divate AD. Fruit wine production: A review. Journal of Food Technology. 2014;2(3):93-100.

Mohagheghi A, Maghsoud S, Khashayar P, Ghazi-Khansari M. The effect of Hibiscus sabdariffa on lipid profile, creatinine and serum electrolytes: A randomized clinical trial. ISRN Gastroenterology. 2011;976019.

DOI: 10.5402/2011/976019

Singh P, Khan M, Hailemariam H. Nutritional and health importance of Hibiscus sabdariffa: A review and indication for research needs. Journal of Nutritional Health & Food Engineering. 2017;6(5):00212.

Riaz M, Zia-Ul-Haq M, Saad B. Anthocyanins and human health: Biomolecular and therapeutic aspects. Switzerland: Springer. 2016;125-138.

Hassan HA, Abdel-Aziz AF. Evaluation of free radical-scavenging and anti-oxidant properties of black berry against fluoride toxicity in rats. Food and Chemical Toxicology. 2010;48(8-9):1999-2004.

Sancho RAS, Pastore GM. Evaluation of the effects of anthocyanins in type 2 diabetes. Food Research International. 2012;46(1):378-386.

Ellingsen I, Hjerkinn EM, Seljeflot I, Arnesen H, Tonstad S. Consumption of fruit and berries is inversely associated with carotid atherosclerosis in elderly men. British Journal of Nutrition. 2008;99(3): 674-681.

Haripyaree A, Guneshwor K, Damayanti M. Evaluation of antioxidant properties of phenolics extracted from Ananas comosus L. Notulae Scientia Biologicae. 2010;2(2): 68-71.

Ahmad I, Chwee CP. An overview of the world production and marketing of tropical and subtropical fruits. In International Workshop on Tropical and Subtropical Fruits. 2007;787:47-58.

Hossain MF, Akhtar S, Anwar M. Nutritional value and medicinal benefits of pineapple. International Journal of Nutrition and Food Sciences. 2015;4(1):84-88.

Hossain MA, Al-Toubi WA, Weli AM, Al-Riyami QA, Al-Sabahi JN. Identification and characterization of chemical compounds in different crude extracts from leaves of Omani neem. Journal of Taibah University for Science. 2013;7(4):181-188.

Alashi AM, Blanchard CL, Mailer RJ, Agboola SO, Mawson AJ, He R, Aluko RE. Antioxidant properties of Australian canola meal protein hydrolysates. Food Chemistry. 2014;146:500-506.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9-10): 1231-1237.

Van den Berg R, Haenen GR, van den Berg H, Bast AALT. Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chemistry. 1999;66(4): 511-517.

AOAC. Official methods of analysis (22nd Edn). Association of Official Analytical Chemists. Washington USA; 2005.

Harrigan WF. Laboratory methods in food microbiology. Gulf Professional Publishing; 1998.

Iwe MO. Handbook of sensory methods and analysis. Rojoint Communication Services Ltd., Enugu, Nigeria. 2002;71-75.

Gómez-Plaza E, Gil-Muñoz R, López-Roca JM, Martínez-Cutillas A, Fernández-Fernández JI. Phenolic compounds and color stability of red wines: Effect of skin maceration time. American Journal of Enology and Viticulture. 2001;52(3):266-270.

Jagtap UB, Waghmare SR, Lokhande VH, Suprasanna P, Bapat VA. Preparation and evaluation of antioxidant capacity of Jackfruit (Artocarpus heterophyllus Lam.) wine and its protective role against radiation induced DNA damage. Industrial Crops and Products. 2011;34(3):1595-1601.

Joshi VK, Sharma S, Thakur AD. Wines: White, red, sparkling, fortified and cider. In Current Developments in Biotechnology and Bioengineering. 2017;353-406.

Margalit Y. Concepts in wine chemistry. Board and Bench Publishing; 2012.

Iwegbue CM, Nwozo SO, Overah CL, Bassey FI, Nwajei GE. Concentrations of selected metals in some ready-to-eat-foods consumed in Southern Nigeria: Estimation of dietary intakes and target hazard quotients. Turkish Journal of Agriculture-Food Science and Technology. 2013;1(1):1-7.

Thurmond DL. Vinification. In From Vines to Wines in Classical Rome. 2017;136-207.