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The aim of the present research was to assess plasma selenium concentrations in healthy individuals, in the State of Mato Grosso do Sul, Brazil, as part of the mapping of the Brazilians states. Selenium levels were measured in 342 healthy individuals, using Inductively Coupled Plasma Spectrometry (ICP-OES). The average plasma selenium concentration in this study was 65±20 μg/L (mean±SD; range 21– 144 μg/L), the lowest in comparison to other Brazilian states. The predictor variables, such as gender, ethnicity, blood type or body mass index do not correlate with plasma selenium concentration.